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    • 专利摘要:
    The present invention relates to a beer with an ethanol content of 0-1.0% by volume, comprising at least 0.001 µg / l ethyl-2-methylpentanoate (EMP). EMP has been found to have a strong wort flavor masking effect. A beer according to the invention has a low root flavor and less predominant taste and is usually considered refreshing.
    公开号:BE1026598B1
    申请号:E20195601
    申请日:2019-09-10
    公开日:2020-04-09
    发明作者:Eric Richard Brouwer；Hilda Els Smit；Augustinus Cornelius Aldegonde Petrus Albert Bekkers；Albert Doderer
    申请人:Heineken Supply Chain Bv；
    IPC主号:
    专利说明:

    Low alcohol beer with reduced wort flavor
    The present invention relates to an ester with particularly good wort flavor masking properties, which has proved to be an important contributor to beer flavor.
    Background
    Beer is one of the most popular alcoholic drinks worldwide. It is prepared by fermentation of a sugary aqueous matrix derived from grains, using yeast that converts the sugars into ethanol ("alcohol"). The beer production process is well known. Beer is usually made from grain such as barley, although other types of grain such as wheat or sorghum can also be used. Beer is usually produced by a process that includes the following basic steps: mashing a mixture of grain and water to produce a mash; separating the mash into wort and brewing; cooking the wort to produce a boiled wort; fermenting the cooked wort with live yeast (such as Saccharomyces pastorianus or Saccharomyces cerevisiae) to produce a fermented wort; subjecting the fermented wort to one or more further process steps (e.g. maturation and filtration) to produce beer; and packaging the beer in a sealed container, e.g. a bottle, can or barrel.
    In an exemplary process to produce a barley malt beer, the barley is malted, which means that it is germinated and then dried ('eesten') to produce malt. This process is important for the formation of flavor and color compounds, and the formation of enzymes that are important for further flavor development and starch breakdown. The malt is then ground and suspended in water ('mashing'). The maize is heated to facilitate starch breakdown. Subsequent filtration results in wort, which is a more or less clarified aqueous solution of fermentable sugars, which is also
    BE2019 / 5601 contains various flavors and aromas and many other compounds. Both desirable and undesirable flavor and aroma compounds are present in wort. An exaggerated 'wort-like' flavor, due to the excessive presence of aldehydes, is usually considered undesirable.
    The wort is boiled to sterilize it, to precipitate proteins and to concentrate it. Hops are optionally added to add bitterness and flavor. After removal of the precipitant, this mixture is subjected to fermentation. Fermentation results in the conversion of fermentable sugars into ethanol and carbon dioxide, and also results in the formation of various new flavor compounds, including esters. At the same time, fermentation of beer removes most of the aldehydes, preventing a wort-like taste from the resulting beer. After fermentation, the beer can be filtered and / or stored to optimize appearance and taste.
    Health concerns and increased road safety awareness associated with the alcohol content of beer have heightened interest in low-alcohol or even non-alcoholic beer. Currently, there are two main techniques for the production of low-alcohol or non-alcoholic beer: de-alcoholization of regular (alcoholic) beer, and preparation of beer by a method that limits the formation of alcohol through appropriate fermentation (“limited alcohol fermentation ”).
    De-alcoholization of beer is carried out on regular brewed beer, and is intended to remove ethanol, but as few other flavor components as possible. De-alcoholization can be achieved by, for example, rectification, reverse osmosis or dialysis of regular beer. However, it is a challenge to prevent loss of flavor in the de-alcoholization of beer. A disadvantage of de-alcoholized beer is therefore a bland taste, which can be corrected by adding flavors (taste and smell) in order to obtain an acceptable beer. However, because taste is complex due to the large
    BE2019 / 5601 variety of compounds which together are responsible for imparting flavor, de-alcoholized and subsequently flavored beer is generally considered less pleasant in taste than the taste of regular beer.
    Low-alcohol and non-alcoholic beer can also be prepared through limited alcohol fermentation. Limited alcohol fermentation is a process in which wort is fermented under conditions where there is little or no ethanol formation (or at least, where the entire fermentation process results in little or no net alcohol formation).
    An important method is cold-contact fermentation. When wort is fermented at a low temperature, yeast hardly produces alcohol, although it does produce a number of flavor components such as esters, although amounts per ester may differ from the amounts obtained from regular fermentation. Therefore, for beer with limited fermentation, taste correction by adding flavors and aromas is generally necessary. Also such beers are usually considered less pleasant in taste than regular beer.
    Existing low-alcohol or non-alcoholic beers generally suffer from a lack of drinkability. Most people get satiated by the taste after just one or two glasses, as opposed to drinking regular alcoholic beer. The taste saturation and the resulting lack of drinkability is generally caused by a predominant taste caused by too intense wort-like flavors due to high aldehyde levels and too high sweetness. The present invention provides a method to overcome these drawbacks.
    Summary of the invention
    The invention discloses a beer with an ethanol content of 0 - 1.0% by volume, comprising at least 0.001 µg / l ethyl 2-methylpentanoate (EMP). EMP has been shown to be a major contributor to beer flavor.
    BE2019 / 5601
    EMP is effective in masking the wort taste and thereby suppresses the wort-like taste. This is particularly important with low-alcohol or non-alcoholic beer, as such beers generally suffer from wort-like flavor due to increased aldehyde presence over regular brewed beer. In addition, the beer is usually considered less thick, and more refreshing. For this reason, the present beer is considered more drinkable than existing commercial non-alcoholic beers.
    Detailed description
    In the present context, beer is to be understood in a broad sense, that is, beer can refer to any type of beer, including but not limited to ale, porter, stout, lager and bock beer. Beer is preferably a malt-based beer, that is, a beer prepared from the fermentation of wort prepared from (inter alia) malt. Preferably, beer is lager, which is a beer obtained by fermentation at 7-15 ° C using a bottom yeast, and subsequent storage at low temperature. Lager, for example, includes pilsner. Most preferably, a beer as described herein is a pilsner. A pilsner is a light lager. It is an object of the invention to provide a low-alcohol or non-alcoholic beer with the taste and drinkability of a regular beer.
    In the present text, "non-alcoholic or low-alcohol beer" is a beer with an ethanol content of 1.0% by volume ("ABV") or less, preferably 0.5% by volume or less, more preferably 0.2% by volume. % or less. Such beers are also called non-alcoholic (NA) beers. NA beer is thus a beer with an ethanol content of 0 - 1.0% by volume, such as preferably 0 - 0.5% by volume.
    The invention discloses an NA beer, with an ethanol content of 0-1.0 vol.%, Preferably 0-0.5 vol.%, Comprising at least 0.001 µg / l ethyl 2-methylpentanoate (EMP). An NA beer can be obtained by well known methods.
    One method of obtaining an NA beer as defined herein is by subjecting a regularly brewed beer to a de
    BE2019 / 5601 alcoholization step, such as, for example, a rectification step, a reverse osmosis step, a dialysis step or a freezing concentration step, to remove ethanol from the fermented beer. These techniques are described, for example, in Brânyik et al, J. Food. Spooky. 108 (2012) 493-506, or in Mangindaan et al, Trends in Food Science & Technology 71 (2018) 3645.
    De-alcoholization of regular beer results in a de-alcoholized beer, which is one type of NA beer as defined herein. Regular beer is, in the present context, regular brewed beer, with an ethanol content of 1 - 15% by volume, preferably 3 - 9% by volume. The regular beer is preferably regular lager. The skilled artisan is able to obtain regular beer, including regular lager, for example, by the methods described in The Brewer's Handbook (second edition) by Ted Goldammer (2008, Apex Publishers). Alternatively, regular beer can be obtained commercially. It has now been found that regular beer inherently contains EMP, which forms under regular (non-limited) conditions during fermentation.
    De-alcoholization of regular beer results in beer with a low amount of ethanol, but also results in the removal of many esters and other compounds responsible for the taste of the beer. Such beers can be flavored prior to consumption to impart flavor to the de-alcoholized beer. Such flavors do not include EMP, since the importance of EMP as a component in beer flavoring has not yet been recognized.
    In the present context, a de-alcoholized beer is a preferred type of NA beer. Furthermore, the NA beer in the present context is preferably a de-alcoholized beer obtained by correction.
    Another method of obtaining NA beer is to make beer by a limited fermentation process, which is a limited beer
    BE2019 / 5601 yields fermentation. A limited fermentation beer is another type of NA beer as defined herein.
    A beer with limited fermentation is defined as a fermented beer obtained by limited ethanol fermentation of wort. Limited ethanol fermentation of wort is fermentation that does not result in significant net ethanol formation, that is, limited fermentation as defined herein results in 1 vol% or less, preferably 0.5 vol% or less ethanol, more preferably 0, 2 vol.% Or less. Thus, a beer with limited fermentation has an ethanol content of 1.0% by volume or less, preferably 0.5% by volume or less, more preferably 0.2% by volume or less.
    Limited wort fermentation is a process in which the product obtained directly from the fermentation has an ethanol content of 1.0 vol% or less, preferably 0.5 vol% or less, more preferably 0.2 vol% or less . Those skilled in the art are aware of various limited fermentation techniques that do not result in significant net ethanol formation. Examples are limited ethanol fermentation of wort characterized by • a temperature below 7 ° C, preferably -1-4 ° C, such as -0.5 - 2.5 ° C, preferably over a period of 8 - 72 hours, with more preferably 12 - 48 hours ('cold-contact fermented beer'); and / or • a short (eg less than 2 hours) fermentation time, which fermentation quickly stopped by temperature inactivation, such as by rapid cooling to -0.5 - 1 ° C, optionally followed by subsequent pasteurization ('beer with stopped fermentation' ); and / or • fermentation by a yeast strain producing relatively low amounts of ethanol under the fermentation conditions employed, such as, for example, a yeast strain producing less than 0.2 g ethanol per gram fermentable sugar in the wort, preferably less than 0.1 g ethanol per grams of fermentable sugar. Suitable strains (eg Crabtree
    BE2019 / 5601 negative strains) are known in the art and the amount of ethanol produced under varying fermentation conditions can be determined by routine experiments ("yeast-limited beer"); and / or • fermentation using a first ethanol-producing yeast strain, in the presence of a sufficient amount of a second yeast strain that consumes ethanol, such as Saccharomyces rouxii, to consume substantially all ethanol produced by the first yeast strain; and / or • wort with a content of fermentable sugars, such that a maximum of 1.0% by volume of alcohol is produced upon completion of its fermentation. In this case, the wort usually has a fermentable sugar content of less than 17.5 g / l, preferably less than 12 g / l, more preferably less than 8 g / l ("low sugar worthier").
    A beer with limited fermentation has not been subjected to a deal alcoholization step to obtain said ethanol content of 1.0 vol.% Or less, preferably 0.5 vol.% Or less, more preferably 0.2 vol.% Or less. The skilled artisan knows various suitable techniques for de-alcoholization of a fermented beer, and none of these techniques has been used to achieve the said ethanol content. However, a beer with limited fermentation in the present context can optionally be subjected to a de-alcoholization step to reduce the ethanol content of said 1.0% by volume or less, preferably 0.5% by volume, more preferably 0% 2% by volume or less, as obtained from the fermentation, to a further reduced ethanol content. Preferably, however, a beer with limited fermentation as defined herein is not subjected to a de-alcoholization step at all.
    A de-alcoholization step for further reducing the ethanol content of a beer with limited fermentation of 1.0% by volume or less as defined above to even lower amounts of ethanol is in
    BE2019 / 5601 well known in the art. This has been described above, with exemplary reference to Mangindaan et al and Brânyik et al.
    A limited fermentation beer in the present context is preferably a low-sugar worthier, a yeast-limited beer, a stop-fermented beer, or a cold-contact fermented beer. In one embodiment, the limited fermentation beer is a low-sugar worthier. In a further embodiment, the limited fermentation beer is a yeast-limited beer. In yet a further embodiment, the limited fermentation beer is a stopped fermentation beer. In yet a further embodiment, the limited fermentation beer is a cold-contact fermented beer. In preferred embodiments, the limited fermentation beer is a cold-contact fermented beer. This is because cold-contact fermented beer contains relatively higher amounts of wort-like flavors than the other types of beer with limited fermentation (see Branyik et al, cited above).
    Cold contact fermentation is well known in the art and those skilled in the art can obtain a cold contact fermented beer by any means known or known in the art. An exemplary method of obtaining a cold-contact fermented beer is described, for example, in Branyik et al, cited above. Alternatively, cold-contact fermented beer can be obtained commercially.
    Although limited fermentation can result in the formation of some ester compounds, the amount and type of ester differs from the ester mix found in regular brewed beer. For this reason, the taste is less pleasant, which is corrected by supplementing a suitable flavoring agent to give the desired taste. It has now been found that limited fermentation does not result in significant EMP formation.
    EMP has been found to be a component in regular brewed beer that is important for providing beer flavor. This component is present in significantly lower amounts in known zero or low beer
    BE2019 / 5601 alcohol, because of low formation in a process with limited fermentation, including a cold-contact process, and / or because of reduced amounts that are present after dealcoholization.
    An important function of EMP is to mask wort flavor. In addition, EMP provides a fruity, apple-like taste and results in the refreshing character associated with regular beer. For these reasons, it has been found beneficial to enrich NA beer with EMP, such as in an amount of at least 0.001 pg / l ('ppb'). Accordingly, the invention further discloses the use of ethyl 2-methyl pentanoate to mask root flavor and / or provide refreshing flavor.
    The amount of EMP in the NA beer is preferably at least 0.004 pg / l, more preferably at least 0.01 pg / l, even more preferably at least 0.1 pg / l. Preferred amounts of EMP include 0.1-1000 pg / L, preferably 1-800 pg / L, more preferably 10-500 pg / L. In preferred embodiments, the amount of EMP is 50-600 pg / l.
    An important effect of EMP is to mask the wort-like taste commonly present in NA beer, especially cold-contact fermented beer. Nevertheless, it is preferable if the beer contains relatively low amounts of aldehyde. In preferred embodiments, the total aldehydes in the beer, defined as the total of 2-methylpropanal, 2-methylbutanal, 3methylbutanal, 3-methylthiopropionaldehyde, phenylacetaldehyde, hexanal, trans-2-nonenal, benzaldehyde and furfural, are less than 600, preferably less more than 400, more preferably less than 200, most preferably less than 80 pg / l. In further preferred embodiments, the total of Strecker aldehydes (defined as the total of 2methylpropanal, 2-methylbutanal, 3-methylbutanal, 3methylthiopropionaldehyde and phenylacetaldehyde) is less than 200, at
    BE2019 / 5601 preferably less than 150, more preferably less than 100, most preferably less than 50 pg / l.
    The amount of EMP in NA beer with these aldehyde amounts is at least 0.001 pg / l, preferably at least 0.004 pg / l, more preferably at least 0.01 pg / l, even more preferably at least 0.1 pg / l such as 0.1-1000 pg / l, preferably 1-800 pg / l, more preferably 10-500 pg / l. In preferred embodiments, the amount of EMP is 50-600 pg / l. It is preferred that when higher amounts of aldehydes are present, the amount of EMP is also higher.
    It is preferred if the non-alcoholic or low-alcohol beer is not very sweet because it improves drinkability. The total sugar content of the obtained NA beer, defined as the total glucose, fructose, sucrose, maltose and maltotriose, is preferably at least 0.2 g / 100 ml. It is preferred if the total sugar content is relatively low, such as at most 3 g / 100 ml, preferably at most 2 g / 100 ml. In preferred embodiments, the total sugar content is 0.5-2.0 g / 100 ml, preferably 1.2-2.0 g / 100 ml.
    The beer obtained preferably comprises at least 0.2 g / 100 ml of maltotriose. Preferably, the beer further comprises at least 0.05 g / 100 ml of glucose and / or at least 0.05 g / 100 ml of fructose. It is further preferred if the total sugar content of the beer obtained comprises 50-100 wt% maltose, preferably 50-80 wt%, more preferably 50-65 wt%. The maltose content of the final beer is preferably at least 0.5 g / 100 ml.
    A preferred beer obtained by the present method further comprises 20-250 mg / l free amino nitrogen [FAN) (nitrogen), more preferably 50-200 mg / l, more preferably 75-150 mg / l. Free amino nitrogen, as used herein, refers to the total amount of free amino compounds as determined by the NOPA method. This method results in quantification of primary amino compounds,
    BE2019 / 5601 such as free amino acids, small peptides and ammonia. The amount of FAN listed is an important aspect of the final beer taste and color.
    A preferred beer obtained by the present method further comprises less than 5 mg / l acetaldehyde, preferably less than 3 mg / l. This is important for the flavor profile of the beer obtained.
    A beer according to the invention preferably also comprises 1 - 20 µg / l, preferably 1.5-5 µg / l ethyl propanoate, which is important for flavoring. A beer according to the invention preferably further comprises 0.05 - 30 mg / l ethyl acetate, preferably 0.1 - 15 mg / l ethyl acetate. This is also important for adding flavor. The beer obtained from the distillation further preferably comprises isoamyl acetate in an amount of 0.05 - 7.5 mg / 1, preferably 0.08 - 4.5 mg / 1, more preferably 0.1-1 mg / 1 .
    Advantages of the present beer include improved flavor characteristics, including reduced sweetness and reduced wort-like taste. This results in, among other things, a less predominant taste. The present beer has low sweetness and favorable aldehyde levels, even with respect to this reduced sweetness. As a result, at a lower sweetness and flavor intensity, the beer is not considered to be wort-like, and consumers do not become saturated with the taste after just a few beers. In addition, the beer is usually considered less thick, and more refreshing. Thus, a beer of the present invention is more drinkable than existing zero alcohol beers.
    The invention further discloses a method for preparing a beer with an alcohol content of 0-1.0 vol.%, Preferably 0 - 0.5 vol.%, Comprising a combining step of a beer with zero or low alcohol as defined above with ethyl 2-methylpentanoate. In one embodiment, the EMP can be combined with the NA beer by addition as a flavoring agent. In such embodiments, the EMP can be obtained commercially, and / or it can be obtained by
    BE2019 / 5601 insulation from a natural source, such as by insulation from regularly brewed beer. Thus, in preferred embodiments, the combination with ethyl 2-methyl pentanoate is accomplished by combination with a flavoring agent comprising ethyl 2-methyl pentanoate.
    In one embodiment, the flavoring agent is flavoring agent comprising ethyl 2-methylpentanoate. In this embodiment, the flavoring agent may comprise a flavor mix designed to achieve specific amounts of esters in the final beer. The flavoring agent may also include other flavoring compounds and flavors, such as (organic) acids, dialdehydes, higher alcohols, salts and the like.
    In other embodiments, the flavoring agent may be a regular beer, preferably regular lager, more preferably a pilsner. An advantage of using regular beer as a flavoring agent is that regular beer comprises a mix of flavors and aromas that are considered natural by a beer consumer, resulting in an NA beer with a regular beer-like flavor and aroma profile.
    In some embodiments, a regular beer flavor may be a flavor derived from regular beer by concentration, such as by partial or complete removal of alcohol and / or water to provide a regular beer concentrate, which may be in solid or liquid form. Suitable techniques for obtaining such a beer concentrate are, for example, freeze drying and freeze concentration, although the skilled person can think of numerous ways to obtain a suitable beer concentrate using his current general knowledge.
    In one embodiment, the flavoring agent is regular beer, preferably regular lager. Preferably, the method in this embodiment comprises a step of mixing beer with limited fermentation, preferably cold contact fermented beer, with regular beer, preferably regular lager. It is preferred as the beer with
    BE2019 / 5601 limited fermentation and the regular beer are mixed in a volumetric ratio of 1: 99-99: 1, preferably 5:95 - 50:50.
    In some embodiments, the mixing step is followed by vacuum distillation to remove excess aldehydes from the beer with limited fermentation. In such embodiments, it may be an advantage to add a further flavoring agent, for example a flavoring agent comprising a flavor mix as defined above, after said distillation step to achieve a desired flavor profile.
    For the purpose of clarity and brief description, features are described herein as part of the same or separate embodiments; however, it will be appreciated that the scope of the invention may include embodiments with combinations of all or some of the features described.
    The invention will now be further illustrated with the following non-limiting examples.
    Methods
    Determination of aldehydes in beer using on-fiber derivatization, solid phase microextraction and gas chromatography mass spectrometry.
    Sample preparation
    Under a CO2 atmosphere, 30.0 g of beer sample was weighed into a 40 ml bottle. With a 50 μΐ gastight syringe, 30 μΐ of internal standard solution was added to each sample. Then, still under CO2 conditions, two 10 ml gas-chamber bottles were filled with 4.0 g of the sample. Thus, the beer sample is analyzed in duplicate.
    BE2019 / 5601
    Derivatization procedure
    A stock solution of about 200 mg / 1 O- (2,3,4,5,6-pentafluorobenzyl) -hydroxylamine (PFBHA) in deionized water was prepared. A standard 20 ml clear glass crimp-cap gas-clearing vial was charged with 14 g of the PFBHA solution. Then, an SPME fiber (Supelco) was placed in the gas chamber of the derivatization vial for 10 minutes at 30 ° C to impregnate the PDMS / DVB phase of the SPME fiber with the derivatizing agent. The SPME fiber infused with PFBHA was then placed in the gas space of a beer sample (4.0 g in 10 ml vial, filled under a CO2 atmosphere) leading to PFBHA derivatives of the aldehydes of interest. The extraction conditions were chosen at 30 ° C for 30 minutes.
    GC conditions
    An Agilent 7890A gas chromatograph, equipped with a “split-splitless” inlet, was used for this method. Optimal separation of the PFBHA / aldehyde compounds was achieved with a 30 m x 0.25 mm x 0.25 μm VF17MS column (Agilent). Some compounds co-elute, but in these cases the selectivity of the MS could circumvent the need for chromatographic separation. For example, trans-2-nonenal, phenylacetaldehyde and decanal all co-elute, but the mass spectrometer focuses on selective ions for each compound.
    Helium at 1 ml / min is used as the carrier gas. A split ratio of 5 used to optimize peak width and sensitivity. The oven was programmed to 50 ° C (2 minutes) with a 10 ° C / min rise to 230 ° C, followed by a 30 ° C / min rise to 290 ° C (2 minutes hold).
    BE2019 / 5601
    MS conditions
    The Agilent 5975C MSD was set for negative chemical ionization (NCI).
    For all aldehydes targeted by this methodology, a suitable ion fragment was chosen. In most cases, a compound-specific fragment was chosen rather than the most common fragment. This allows the selective determination of the target compounds of interest. In most cases, the molecular ion Mw minus m / z 20 (loss of H-F) is most suitable.
    Since most of the PFBHA aldehyde compound consists of two peaks (syn and anti), the peak areas are added. Quantification of all aldehyde compounds was possible by setting appropriate calibration curves based on standard Heineken beer addition.
    Determination of ester and alcohol bonds in beer using Stir Bar Sorptive Extraction and gas chromatography - mass spectrometry
    Due to the large concentration range of the analytes, the samples must be analyzed twice using different GCMS methods; one for the determination of compounds with high concentrations, and one for the compounds with low concentrations. The difference between the two methods is the mode of operation of the mass selective detector. Isoamyl acetate was determined using the high concentration method, and ethyl acetate was determined using the low concentration method.
    Sample preparation
    In duplicates, 30.0 g of beer sample was weighed into a 40 ml bottle. Using a 50 μ inject gastight syringe, 30 μΐ of internal was taken
    BE2019 / 5601 standard solution added to each sample. A pure and already conditioned twister was then added to each sample. A 60-position stir plate was used to extract the samples. Extraction time is one hour. In order to prevent the formation of light-induced compounds, the samples are covered with light.
    Instrument conditions
    The Agilent 7890B gas chromatograph is coupled to an Agilent 5977A MSD. Sample entry is performed by a Gerstel MPS2-XT robot, in combination with a TDU-2 thermal desorption unit and CIS-4 controlled temperature inlet (both from Gerstel). The capillary main column is a 50m x 0.25mm x 0.25pm DB-5MS UI. Helium at 1.5 ml / min is used for carrier gas. The thermal desorption of the twisters was performed in solvent vent mode, which collects the eluents at -20 ° C on a tenax filled liner. During the heating step, the injector switched to a split ratio of 100: 1 and 6: 1 for the high and low concentration process, respectively. The oven was programmed at 40 ° C (2 minutes) with a 10 ° C / min rise to 280 ° C (1 minute hold). The MSD is set for scan mode (33-300 m / z) in the high concentration method, and in single ion mode for the low concentration method.
    Quantification of all compounds was made possible by the preparation of calibration curves (standard addition to a suitable matrix beer). All calibrations are performed in both alcoholic and 0.0% alcoholic drinks.
    Determination of sugar content in wort, beer and cider
    Sugar content was measured by Ultra Performance Liquid Chromatography (UPLC). UPLC can be conveniently performed at a
    BE2019 / 5601 temperature of 65 ° C. A suitable choice for the eluent is a mixture of acetonitrile / water, for example in a volume ratio of 75/25. The detector used is typically a Refractive Index (RI) detector. The sugar content of a sample was determined by comparing the UPLC curve of the sample with calibration curves of standard samples with known sugar concentrations.
    The samples for UPLC were prepared as follows. A beer or wort sample was diluted a factor of 5 by adding acetonitrile / water mixture (50/50 - equal parts by volume). If present, CO2 was removed prior to dilution (e.g., by shaking or stirring the sample). After dilution, the sample was filtered to obtain a clear solution. The filtered sample was injected into the UPLC at 65 ° C using the above eluent.
    Determination of free amino nitrogen (FAN)
    The amount of free amino nitrogen was measured by the Nitrogen by O-Phthaldialdehyde Assay (NOPA) method. The NOPA method was performed using a photometric analyzer (eg, Gallery ™ Plus Photometric Analyzer). According to the NOPA method, a test sample is subjected to treatment with ortho-phthalaldehyde [ortho-phthalaldehyde] (OPA) and N-acetylcysteine (NAC). This treatment results in the derivatization of primary amino groups present in the test sample to form isoindoles. The content of the isoindoles is then determined using the photometric analyzer at a wavelength of 340 nm. The free amino nitrogen (expressed in mg FAN / 1) can then be calculated based on the measured content of the isoindoles. If necessary, the beer or wort sample may be centrifuged prior to analysis
    BE2019 / 5601 sample and / or subjected to a CO2 removal step (e.g. by stirring or shaking the sample).
    Determination of ethanol in beer
    The ethanol content was measured using a photometric analyzer (eg, Gallery ™ Plus Photometric Analyzer). The test sample is subjected to an enzymatic method, in which the ethanol contained in the sample is converted into acetaldehyde with alcohol dehydrogenase (ADH). The acetaldehyde content is then determined using the photometric analyzer at a wavelength of 340 nm. The ethanol content can be calculated on the basis of the acetaldehyde content. If necessary, the beer or wort sample is first centrifuged before analysis to clarify the sample and / or subjected to a CO2 removal step (e.g. by stirring or shaking).
    Example 1
    The effect of ethyl 2-methylpentanoate as a wort flavor mask and providing a refreshing fruity beer flavor.
    An alcohol-free beer was prepared by rectification. The resulting blank beer was relatively low in flavors such as esters. Ethyl 2-methylpentanoate (EMP) was added to the blank beer as a flavoring agent at 50 ppb. There were no further differences between the blank beer and the fortified beer. The sensory differences between the blank beer and the EMP-enriched beer were indicated by a trained sensory panel of 17 persons. The sensory panel indicated the different taste attributes between the two beers. The number of panel members who reported a higher presence of flavor attributes is presented in Table 1.
    BE2019 / 5601
    From the results, it can be seen that EMP has a particularly strong wort flavor masking effect. Wort flavor (which comes from aldehydes) was considered to be more abundant in the blank beer by 16 of the 17 panelists. In the blank beer enriched with 50 µg / l EMP, the wort flavor was suppressed. 50 µg / l EMP therefore suppresses the wort taste to a great extent.
    In addition, the presence of EMP provides an acidic, fruity and citrus flavor. Thus, the enriched beer becomes more refreshing
    considered. Table 1 Blank beer Blank beer + 50 pg / l EMP Bitter 5 6 Sweet 8 4 Acid 3 7 Predominantly fruity 0 16 Citrus / refreshing 0 11 Wort 16 0 Metallic 10 2 Sulfur 12 0 Astringent 5 0 Isovaleric acid 1 7 Diacetyl 0 2 Herbaceous 4 0 Seductive 1 0 Thin 0 1
    Example 2
    The effect of ethyl 2-methylpentanoate as a wort flavor mask and as a provider of a refreshing fruity beer flavor in fully flavored beer.
    A zero alcohol beer obtained by vacuum distillation of a mixture of regular beer and cold-contact fermented beer, and fully flavored to provide beer flavor using a flavor mix that did not include EMP, was enriched with increasing amounts of EMP (1, 50, 250, 500 and 1,000 ppb EMP). A taste panel
    BE2019 / 5601 consisting of 17 trained taste experts scored the perceived intensity for 3 taste attributes: wort, fruitiness and apple flavor. The results are shown in Figure 1.
    The results show that increasing the amount of EMP in the alcohol-free or low-alcohol beer increasingly masks the wort-like flavors and increases the fruit flavor. In the context of a flavor mix, EMP gives an apple-like taste and provides a strong refreshing effect.
    Example 3
    Comparison of the beer obtained with commercially available non-alcoholic and low-alcohol beers
    A beer obtained using the present method was compared to existing non-alcoholic beers by a taste panel of 17 trained panelists. Beers were evaluated for specific taste characteristics, including sweetness, wort flavor and total flavor intensity, as well as other flavor toes. For each characteristic, the observed intensity was indicated on a line scale. All panelists evaluated all beers for all characteristics, and results were averaged. The results are shown in Table 2.
    A beer obtained by the present method has a less wort-like taste and less sweet taste than commercially available non-alcoholic beers. The likelihood that consumers will become saturated with the taste in just a few beers is lower, because the taste is less predominant. In addition, the beer is generally considered to be less thick, and more refreshing. For this reason, the present beer is considered to be more drinkable than existing commercially available non-alcoholic beers.
    BE2019 / 5601
    Commercially available beer E T — 1 38Γχΐ T — 1 cn T — 1 O36 29 cn LH Commercially available beer D 23 33 20 LH Γχΐ Γχΐ ττ T — 1 43 m 28 Lf) 23 Commercially available beer C 23 28 33 Γχΐ Ξ Lf) 24 Lf) 10 32 29 Lf) 23 Commercially available beer B 26 r i 00 Γχΐ r ».00 O Lf) 35 32 Γχΐ 25 Commercially available beer A 28 39 T — 1 2 Lf) Γχΐ O T — 1 44 33 32 Lf) 25 Current beer 00 (N ΓΌ 29 CD 11 2 Γχΐ 40 42 (N Lf) 00 Table 2 Sweet taste Worts make Fruity / ester-like taste > Pear flavor > Taste of tropical fruit > Taste of green apple Hops make Vinegar flavor Total intensity Refreshing taste in the mouth Thick taste in the mouth Warm taste in the mouth Sweet aftertaste
    BE2019 / 5601
    权利要求:
    Claims (15)
    [1]
    A beer with an ethanol content of 0 - 1.0% by volume, comprising at least 0.001 µg / l ethyl 2-methylpentanoate (EMP).
    [2]
    A beer according to claim 1, wherein the amount of EMP is at least 0.004 µg / l, preferably 0.1-1000 µg / l, more preferably 1-800 µg / l.
    [3]
    A beer according to claim 1 or 2, wherein the total of aldehydes, defined as the total of 2-methylpropanal, 2methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, phenylacetaldehyde, hexanal, trans-2-nonenal, benzaldehyde and furfural less than 600 µg / l, preferably less than 400 µg / l, more preferably less than 200 µg / l, even more preferably less than 80 µg / l.
    [4]
    A beer according to any one of claims 1 to 3, wherein the total of Strecker aldehydes, defined as the total of 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, 3-methylthiopropionaldehyde, and phenylacetaldehyde, less than 200 µg / l is preferably less than 150 µg / l, more preferably less than 100 µg / l, most preferably less than 50 µg / l.
    [5]
    A beer according to any one of claims 1 to 4, wherein the total sugar content, defined as the total of glucose, fructose, sucrose, maltose, and maltotriose, is at least 0.2 g / 100 ml, preferably 0.5 - 2.0 g / 100 ml.
    [6]
    A beer according to any one of claims 1 to 5, wherein the sugar content comprises at least 50% by weight of maltose.
    [7]
    A beer according to any one of claims 1 to 6, further comprising 120 µg / l, preferably 1.5-5 µg / l ethyl propanoate, and / or 0.05-30 mg / l ethyl acetate, preferably 0.1- 15 mg / l ethyl acetate.
    BE2019 / 5601
    [8]
    A method for preparing a beer with an alcohol content of 0 - 1.0% by volume, comprising a step of combining an alcohol-free or low-alcohol beer with ethyl 2-methylpentanoate.
    [9]
    A method according to claim 8, wherein the combination with ethyl 2-methylpentanoate is achieved by combination with a flavoring agent comprising ethyl 2-methylpentanoate.
    [10]
    A method according to claim 9, wherein the flavoring agent is a flavoring mixture comprising ethyl 2-methylpentanoate.
    [11]
    A method according to claim 9, wherein the flavoring is a regular beer, preferably a regular lower beer.
    [12]
    A method according to claim 11, comprising a step of mixing beer with limited fermentation, preferably cold contact fermented beer with regular beer, preferably lower beer.
    [13]
    A method according to claim 12, wherein the limited fermentation beer and the regular beer are mixed in a volumetric ratio of 1:99 - 99: 1, preferably 5:95 - 50:50.
    [14]
    A method according to claim 12 or 13, wherein the mixing step is followed by a vacuum distillation, and optionally further addition of ethyl 2-methylpentanoate.
    [15]
    15. Use of ethyl 2-methylpentanoate to mask wort-like taste and / or provide a refreshing taste.
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    同族专利:
    公开号 | 公开日
    CA3108469A1|2020-03-19|
    CN112673084A|2021-04-16|
    EP3850078A1|2021-07-21|
    BR112021004432A2|2021-05-25|
    JP2022504016A|2022-01-13|
    AR116383A1|2021-05-05|
    NL2023803B1|2020-05-01|
    WO2020055234A1|2020-03-19|
    AU2018441170A1|2021-02-25|
    US20210324309A1|2021-10-21|
    BE1026598A1|2020-04-02|
    CO2021004257A2|2021-04-30|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US5346706A|1991-10-28|1994-09-13|John Labatt Limited|Malt beverage process|
    DE102009026698A1|2009-06-03|2010-12-09|Symrise Gmbh & Co. Kg|Use of certain Isopropylmethylcyclohexenthiole as fragrances and / or flavorings|
    EP2385100A1|2010-05-07|2011-11-09|Anheuser-Busch InBev S.A.|Low alcohol or alcohol free beer and method for producing it|WO2021256542A1|2020-06-19|2021-12-23|サントリーホールディングス株式会社|Beer-flavored beverage|
    法律状态:
    2020-05-29| FG| Patent granted|Effective date: 20200409 |
    优先权:
    申请号 | 申请日 | 专利标题
    PCT/NL2018/050587|WO2020055234A1|2018-09-10|2018-09-10|Low-alcohol beer with reduced wort flavor|
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